Odkazy:
Metody používané pro identifikaci specifické DNA v klinickém materiálu
jsou shodné s následnými citacemi a komerčními kity.
1.Borrelia bugdorferi sensu lato, Borrelia garinii, Borrelia afzelii, Borrelia burgdorferi sensu stricto – průkaz genu 16SrRNA genomové DNA.
Primery BG,VS,BB,LD, z genu 16S rRNA podukt 574,591,574, 356bp teplota annealing 47 C
a)Richard T.Marconi and Claudie F.Garon
Development of Polymerase Chain Reaction Primer Sets for Diagnosis of Lyme Disease and for Species-Specific Identification of Lyme Disease Isolates by 16S rRNA Signature Analysis.
Journal of Clinical Microbiology,Nov.1992,p.2830-2834
Primer 16S ribosom, produkt 169 bp, teplota annealing 55 C
b)Benjamin J.Luft,MD, Charles R.Steiman, MD, Harold C.Neimark,PhD., Bethi Muralidhar,PhD, Thomas Rush, MD, Michael F.Finkel, MD, Mark Kunkel, MD, Raymond J.Dattwyler,MD.
Invasion of the Central Nervous Systém by Borrelia burgdorferi in Acute Disseminated Infection
JAMA,March 11, 1992- Vol.267,No 10, p.1364-1367
2.Borrelia burgdorferi sensu lato gen plasmidové DNA OSPA
OspA-L5 a OspA-R5, produkt 445 bp, teplota annealing 68 C
a)Mark M.Manak,PhD. Lucia I.González-Villaseňor,PhD.,Sylvia Crush-Stanton, and Richard C.Tilton,PhD
Use of PCR Assays to Monitor the Clearance of Borrelia burgdorferi DNA From Blood Following Antibiotic Therapy
Journal of Spirochetal and Tick-borne Diseases, Vol.4, No ½ Spring/Summer 1997 p.11-20
primer OspA 149 a OspA 319(P), produkt 194bp teplota annealing 55 C
US Patent 6087097
b)Andrew R.Pachner, WeiFen Zhang, Henry Schaeffer, Susan Schaeffer and Tim O´Neill
Detection of Active Infection in Nonhuman Primates with Lyme Neuroborreliosis: Comparasion of PCR,Culture, and a Bioassay.
Journal of Clinical Microbiology, Nov. 1998, p.3243-3247
Nested
Primery P66 1.krok produkt 371 bp(inter.kontrola primer PDH 185 bp)tepl. annealing 42C
Primery P66 2.krok produkt 236 bp, tepl. annealing .42 C
c)Susanne Priem, Michael G.Rittig, Thomas Kamradt, Gerd E.Burmester and Andrea Krause,(An Optimized PCR Leads to Rapid and Highly Sensitive Detectionm od Borrelia burgdorferi in Patiens with Lyme Borreliosis.Journal of Clinical Mikrobiology, Mar.1997, p.685-690)
d)Komerční kit pro DNA Borrelia burgdorferi
Genekam Biotechnology AG kat. K018
3.Metody použité k identifikaci DNA Bartonella-specific
primer ITS 16S-23S produkt 670 bp, teplota annealing 50 C
a)J M Rolain, F Gouriet, M Enea, M Aboud, D Raoult (2003)
Detection by immunofluorescence assay of Bartonella henselae in lymph nodes from patients with cat scratch disease.
Clin Diagn Lab Immunol 10: 4. 686-691 Jul
primer ITS 16S-23S produkt 670 bp, teplota 50 Cannealing
b)Jennifer B. Henn,1,5 Mourad W. Gabriel,2,4 Rickie W. Kasten,1 Richard N. Brown,2 Jerold H. Theis,3 Janet E. Foley,4 and Bruno B. Chomel1
Gray Foxes (Urocyon cinereoargenteus) as a Potential Reservoir of a Bartonella clarridgeiae-Like Bacterium and Domestic Dogs as Part of a Sentinel System for Surveillance of Zoonotic Arthropod-Borne Pathogens in Northern California
J Clin Microbiol. 2007 August; 45(8): 2411–2418.
Primer Bh16SF a Bh16SR produkt 185bp, teplota annealing 54 C
c)Edward B.Breitschwerdt, Barbara C.Hegarty and Susan I.HancockSequential Evaluation of Dogs Naturally Infectetd with Ehrlichia canis,Ehrlichia chaffeensis,Ehrlichia equi, Ehrlichia ewingii or Bartonella visonii
Journal of Clinical Microbiology,Sept.1998,p2645-2651 Vol.36,No.9
4.Metody používané k identifikaci DNA Anaplasma phagocytophilum HGE
Primery 593II a ERB2 genu groEL produkt 619 bp, teplota. annealing 65 C
a)Eva Olsson Engvall, Bertil Pettersson, Mariane Persson,Karin Artursson and Karl-Erik Johansson.
A 16S rRNA-Based PCR Assay for Detection and Identification of Granulocytic Ehrlichia Species in Dogs, Horses, and Cattle.
Journal of Clinical Microbiology,Sept.1996,p 2170-2174 Vol.34,No.9
b)Nested
Primer EphplgroEL (569)F a EphplgroEL (1142) R produkt 573bp tep.ann.53 C
Primer EphplgroEL (1193)R a EplgroEL (1084)R produkt 525bp tep.ann.67 C
Equine and Canine Anaplasma phagocytophilum Strains Isolated on the Island of Sardinia (Italy) Are Phylogenetically Related to Pathogenic Strains from the United States.Alberto Alberti, Rosanna Zobba, Bernardo Chessa, Maria Filippa Addis , Oliver Sparagano, Maria Luisa Pinna Parpaglia, Tiziana Cubeddu, Gianpaolo Pitori,and Marco Pittau
Appl.Environ. Microbiol.2005, October, 71(10): 6418-6422
c)Komerční kit k průklazu DNA Anaplasma phagocytophilum
Genekam Biotechnology AG kat. K097
5.Metody používané k identifikaci DNA prvoka rodu Babesia.
primer FOR 1 a REV 1 fragmentu genu 18S rRNA, produkt 1700 bp, teplota annealing .54 C
a)B.Skotarczak, M.Adamska, M.Supron
Blood DNA Analysis for Ehrlichia /Anaplasma)phagocytophila and Babesia spp. Of Dog from Northen Poland
ACTA VET.BRNO 2004, 73,347-351
b)komeční kit pro DNA Babesia gibsoni
firma Genekam Biotechnology AG kat. K022
c) komerční kit pro DNA Babesia bovis
firma Genekam Biotechnology AG kat. K078
6.Metody používané k indentifikaci DNA Rickettsií všech druhů mimo R.helvetica
Primery 120-M59 a 120-807 rOmpB gen, produkt 885bp. annealing .teplota 50 C
a)V.Roux and D.Raoult
Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB(ompB)
International Journal of Systematic and Evolutionary Microbiology (2000) 50,1449-1455
b)Komerční kit pro DNA Ricktettsia rickettsii
Genekam Biotechnology AG, kat. K713
